The Timeline for the Project

The Timeline for the Production of Transgenic Klk1 Deficient Mice

The Isolation of the Gene

09/01/2013 - 11/01/2013

The Generation of The Loxp Vector Construct

11/01/2013 - 01/01/2014

Transformation of the ES cells and the Selection of Transformants

01/01/2014 - 01/11/2014

Transformant Colonies Picked and Expanded

01/11/2014 - 01/25/2014

DNA from Individual Colonies Isolated

01/25/2014 - 02/01/2014

Screening of Colonies Using PCR

02/01/2014 - 02/22/2014

Culturing of ES Cells with Correct Recombinant DNA

02/22/2014 - 02/26/2014

Injection of ES Cells to Blastocysts and Tranfer to Uterus of Surrogate Mother

02/26/2014 - 03/16/2014

Mating of the Transgenic Offspring with FVB/N-Tg Cre Mouse Strain

03/16/2014 - 03/30/2014

Further Mating of the F1 and F2 Progeny

03/30/2014 - 08/28/2014