Pseudoislet Project

Main

Verify ability to make islets in microwells

10/01/2012 - 12/31/2012

Test hypothetic size effect

11/01/2012 - 03/31/2013

Build Standard Protocols

03/01/2013 - 06/15/2013

Indentifying and minimizing the variability
Building DNA assay protocol locally

Long term viability&function of pseudoislets

05/01/2013 - 06/30/2013

Animal Care Certificate training

05/20/2013 - 05/30/2013

In Vitro Test: Small Molecules

05/27/2013 - 10/01/2013

Test a list of cytokines in pseudoislets
(Rock,Caspase...

Hypoxia analysis on pseudoislets

06/01/2013 - 07/31/2013

CFSE Stainning for cell proliferation history

08/01/2013 - 9/30/2013

Animal Care surgical handling training

08/15/2013 - 09/05/2013

L-profile & Cell-subpopulation analysis

10/01/2013 - 12/31/2013

Structural Organization analysis

01/01/2014 - 04/01/2014

Stainning, flow cytometry involved

In Vitro Optimization: cytokines

03/01/2014 - 09/01/2014

Hypoxia analysis

04/01/2014 - 04/30/2014

In Vitro Optimization: other soluble components

07/01/2014 - 10/31/2014

In Vitro Optimization: ECM

10/01/2014 - 01/31/2015

Large scale production of pseudoislets

01/01/2015 - 01/01/2016

In vivo test:mice

05/01/2015 - 12/01/2015

Assesment of survival,engraftment and insulin secretion

09/01/2015 - 12/01/2015

In vivo test: porcine

01/01/2016 - 07/01/2016

Assesment of survival, engraftment&insulin secretion

04/01/2016 - 07/31/2016

Summer Students involvement

Tissue Culture&Microwells Trainning

05/01/2013 - 05/09/2013

Build DNA assay protocol

05/06/2013 - 05/31/2013

Pseudoislets function analysis in more detailed size distribution

05/15/2013 - 08/30/2013

Repetitive work to meet the large throughput in long term viability&function assay

Hypoxia analysis on pseudoislets in size distribution