Lab Timeline

Events

Collected first dirt sample (unsuccessful)

September 11, 2017

Collected second dirt sample (unsuccessful)

September 18, 2017

Collected third dirt sample

October 11, 2017

Phage present after following spot test, however, new phage used for weeks following

New sample (PR3E - Jordandy) from direct isolation

November 27, 2017

Picked a plaque, conducted serial dilutions

Collected lysate from Jordandy

November 29, 2017

Lysate collected from third dilution (10-3)

Performed second serial dilution on Jordandy from lysate

December 4, 2017

To create another lysate

Created second lysate from diluted plate (10^-4)

December 6, 2017

Phage DNA extraction

December 6, 2017

Isolate genomic DNA from phage

Set up restriction enzyme digest

December 13, 2017

Using restriction enzymes: BamHI, Clal, EcoRI, HaeIII, HindIII

Performed gel electrophoresis of restriction enzyme digest

December 15, 2017